My research seeks to understand how the myelin sheath forms during normal axonal development. This knowledge is crucial for understanding demyelinating diseases such as multiple sclerosis. The mechanisms by which the myelin sheath is generated remain elusive. My laboratory colleagues and I are studying the pattern of new membrane addition to forming myelin, and the dynamic movements of protein, lipid and cytoplasmic compartments. We use selected fluorescent-labeled myelin proteins or axonal moieties as reference markers. Our approach studies living, actively myelinating cells observed directly by high-resolution confocal microscopy. We are also using transgenic zebrafish, expressing green-fluorescent-protein (GFP) under the control of a glia-specific promoter, as a model to study in vivo myelination. GFP is expressed in oligodendrocytes and Schwann cells at the time that the myelination program starts, which enables an analysis of the forming myelinating structures in vivo. In addition to trying to understand how myelin forms, we are using this model to study remyelination after laser-ablation of single oligodendrocytes or after chemically induced demyelination. All of our studies are directed at critical, long-standing questions about the sequential events that control the myelination program from both the glial and the neuronal perspective.

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E-mail: Liliana Pedraza